- Claude Bernard -
“The joy of discovery is certainly the liveliest that the mind of man can ever feel”
Publications: Indexed Articles
2020. María Luisa Hernández, Elena Lima-Cabello, Juan de Dios Alche, Jose M. Martinez-Rivas, Antonio J. Castro. Lipid Composition and Associated Gene Expression Patterns During Pollen Germination and Pollen Tube Growth in Olive (Olea europaea L.). Aceptado en Plant and Cell Physiology (2020). Impact Factor (JCR/WOS) 3.929, 23/228 (Q1) Plant Sciences; 80/193 (Q2) Cell Biology (datos de 2018).
2019. Elena Lima-Cabello, Juan D. Alché, Sonia Morales-Santana, Alfonso Clemente, Jose C. Jimenez-Lopez. Narrow-leafed lupin (Lupinus angustifolius L.) seeds gamma-conglutin is an anti-inflammatory protein promoting insulin resistance improvement and oxidative stress amelioration in pancreatic cells. Antioxidants (2019) 2019 Dec 23;9(1). pii: E12. doi: 10.3390/antiox9010012. Impact Factor (JCR/WOS) 4.520, 15/193 (Q1-D1) Food Science & Technology. 6/61(Q1-D1) Chemistry, Medicinal; 63/298 (Q1) Biochemistry&Molecular Biology, DOI: (datos de 2018).
2019 Elena Lima-Cabello, Juan D. Alché, Jose C. Jimenez-Lopez. Narrow-leafed lupin main allergen beta-conglutin (Lup an 1) detection and quantification assessment in natural and processed foods. Foods (2019) Oct 18;8(10). pii: E513. doi: 10.3390/foods8100513. Impact Factor (JCR/WOS) 3.011, 36/135 (Q2) Food Science & Technology. DOI: (datos de 2018).
2019. Maria José Jiménez-Quesada, José Angel Traverso, Martin Potocký, Viktor Zarsky, Juan de Dios Alché. Generation of superoxide by OeRbohH, a NADPH oxidase activity during olive (Olea europaea L.) pollen development and germination. Frontiers in Plant Science10, 1149 (2019). doi: 10.3389/fpls.2019.01149. Impact Factor (JCR/WOS) 4.106, 20/228 (Q1-D1) Plant Sciences. DOI: (datos de 2018).
2019. Estefanía García-Quirós, Juan de Dios Alché, Barbara Karpinska & Christine H. Foyer. Glutathione redox state plays a key role in flower development and pollen vigour. Journal of Experimental Botany erz376 (2019). Impact Factor (JCR/WOS) 5.360, 14/228 (Q1-D1) Plant Sciences. DOI: 10.1093/jxb/erz376 (datos de 2018).
2019. Damián Maestri, Damián Barrionuevo, Romina Bodoira, Adoración Zafra, Jose Carlos Jimenez-Lopez, Juan de Dios Alché. Nutritional profile and nutraceutical components of olive (Olea ueropaea L.) seeds. J. Food Science and Technology. (2019). 56(9): 4359-4370. DOI: 10.1007/s13197-019-03904-5. Impact factor (SJR/Scimago) 0.786 504/2201 (Q1) Agricultural and Biological Sciences. 66/301 (Q1) Food Science.
2019. Sofia Duarte, Tânia Melo, Rosário Domingues, Juan de Dios Alché, Dolores Pérez-Sala. Insight into the cellular effects of nitrated phospholipids: evidence for pleiotropic mechanisms of action. Free Radical Biology and Medicine. 2019. Jun 12. pii: S0891-5849(19)30364-8. doi: 10.1016/j.freeradbiomed.2019.06.003. Impact factor (JCR/WOS) 6.020, 39/293 (Q1) Biochemistry & Molecular Biology, 17/142 (Q1) Endocrinology & Metabolism (datos de 2017).
2019. Agata Kućko, Dariusz Smoliński, Emilia Wilmowicz, Aleksandra Florkiewicz, Juan de Dios Alché. Spatio-temporal localization of LlBOP following early events of floral abscission in yellow lupine. DOI: 10.1007/s00709-019-01365-3. Protoplasma (2019). 256(5): 1173–1183. DOI: 10.1007/s00709-019-01365-3. Impact factor (JCR/WOS) 2.457, 61/223 (Q2) Plant Sciences, 129/190 (Q3) Cell Biology. (datos de 2017).
2019. Elena Lima-Cabello, Isabel Martínez-Beas, Estefanía García-Quirós, Rosario Carmona, M. Gonzalo Claros. Jose Carlos Jimenez-Lopez and Juan de Dios Alché. Structural and functional features of glutathione reductase transcripts from olive (Olea europaea L.) seeds. Lecture Notes in Computer Science Volume (2019) 11465:178-191, 10.1007/978-3-030-17938-0_17. Impact factor (SJR/Scimago) 0.295 (Q2) Computer Science (miscellaneous).
2019. Estefania Sanchez Rodriguez, Sara Biel Glesson, Jose Ramon Fernandez Navarro, Miguel Angel Calleja, Juan Antonio Espejo Calvo, Blas Gil Extremera, Rafael de la Torre, Montserrat Fito, Maria Isabel Covas, Pedro Vílchez, Juan de Dios Alche, Emilio Martinez de Victoria, Angel Gil, Maria Dolores Mesa. Effects of virgin olive oils differing in their bioactive compound contents on biomarkers of oxidative stress and inflammation in healthy adults: A randomized double-blind controlled trial. Nutrients. (2019) 11(3), 561; https://doi.org/10.3390/nu11030561. Impact factor: (JCR/WOS) 4.196, 18/81 (Q1) Nutrition & Dietetics (datos de 2017).
2019 Obando, Melissa; Correa-Galeote, David; Castellano-Hinojosa, Antonio; Gualpa, José; Hidalgo-Garcia, Alba; Alché, Juan de Dios; Bedmar, Eulogio; Cassán, Fabricio: Analysis of the denitrification pathway and greenhouse gases emissions in Bradyrhizobium sp. strains used as biofertilizers in South America. J. Applied Microbiology. 2019. 127(3):739-749. doi: 10.1111/jam.14233. Impact Factor (JCR/WOS) 2.160, 84/161 (Q3) Biotechnology & Applied Microbiology 80/126 (Q3) Microbiology (datos de 2017).
2019 Juan de Dios Alché. A concise appraisal of lipid oxidation and lipoxidation in Higher Plants. Redox Biol. 2019, 2019 Feb 7:101136. doi: 10.1016/j.redox.2019.101136. Impact factor (SJR/Scimago) 7.13, 31/293 (Q1) Biochemistry & Molecular Biology (datos de 2017)
2018. Pedro Seoane, Marina Espigares, Rosario Carmona, Alvaro Polonio, Julia Quintana, Enrico Cretazzo, Josefina Bota, Alejandro Perez-Garca, Juan de Dios Alche, Luis Gomez and M. Gonzalo Claros. TransFlow: A modular framework for assembling and assessing accurate de novo transcriptomes in non-model organisms. BMC Bioinformatics. 2018, 19(Suppl 14):416. DOI:10.1186/s12859-018-2384- Impact factor (SJR/Scimago) 2.511, 9/59 (Q1) Mathematical & Computational Biology, 37/79 (Q2) Biochemical Research Methods, 79/162 (/Q2) Biotechnology & Applied Microbiology.
2018. Adoración Zafra, Mohammed M’rani-Alaoui, Elena Lima, Jose Carlos Jimenez-Lopez, Juan de Dios Alché. Histological features of the olive seed and presence of 7S-type seed storage proteins as hallmarks of the olive fruit development. Frontiers in Plant Science. 2018. 9, 1481. Impact Factor (JCR/WOS) 4.106, 20/228 (Q1-D1) Plant Sciences. DOI: 10.3389/fpls.2018.01481
2018 Elena Lima-Cabello, Sonia Morales-Santana, Josefa León, Víctor Alché, Alfonso Clemente, Juan de Dios Alché and Jose Carlos Jimenez Lopez. Narrow-leafed lupin (Lupinus angustifolius L.) seed β-conglutins reverse back the induced insulin resistance in pancreatic cells. Food & Function. 2018. 9: 5176-5188. DOI: 10.1039/c8fo01164h. Impact Factor (JCR/WOS) 3.241, 31/135 (Q1) Food Science & Technology. 128/298 (Q2) Biochemistry & Molecular Biology.
2018. Pérez-Martín, Fernando; Yuste-Lisbona, Fernando; Pineda, Benito; García-Sogo, Begoña; del Olmo, Iván; Alché, Juan de Dios; Egea, Isabel; Flores, Francisco; Pineiro, Manuel; Jarillo, José; Angosto, Trinidad; Capel, Juan; Moreno, Vicente; Lozano, Rafael. Developmental role of the tomato Mediator complex subunit MED18 in pollen ontogeny. Plant Journal, 96(2):300-315. Doi: 10.1111/tpj.14031 Impact factor (JCR/WOS) 5.726, 11/228 (Q1-D1) Plant Sciences.
2018. Carmona R. Claros M.G, Alché J.D. Bioinformatic Prediction of S-Nitrosylation Sites in Large Protein Datasets. Methods in Molecular Biology. 2018. 1747: 241-250. doi: 10.1007/978-1-4939-7695-9_19. Impact factor (SJR/Scimago)= 0.616, 270/368 (Q3) Molecular Biology. (datos de 2017).
2018 Estefania Sanchez Rodriguez, Elena Lima Cabello, Sara Biel Glesson, Jose Ramon Fernandez Navarro, Miguel Angel Calleja, Maria Roca, Juan Antonio Espejo Calvo, Blas Gil Extremera, Maria Soria Florido, Rafael de la Torre, Montserrat Fito, Maria Isabel Covas, Juan de Dios Alche, Emilio Martinez de Victoria, Angel Gil, Maria Dolores Mesa. Effects of virgin olive oils differing in their bioactive compound contents on metabolic syndrome and endothelial functional risk biomarkers in healthy adults: A randomized double-blind controlled trial. Nutrients. 2018 May 16;10(5). pii: E626. doi: 10.3390/nu10050626. Impact factor: (JCR/WOS) 4.171, 16/86 (Q1) Nutrition & Dietetics.
2018 Zafra A, Castro AJ, Alché JD. Identification of novel superoxide dismutase isoenzymes in the olive (Olea europaea L.) pollen. BMC Plant Biology 2018 Jun 8;18(1):114. doi: 10.1186/s12870-018-1328-z. Impact Factor (JCR/WOS) 3.670, 30/228 (Q1) Plant Science.
2018 Lima-Cabello E, Morales-Santana S, Foley RC, Melser S, Alché V, Siddique KHM, Singh KB, Alché JD, Jimenez-Lopez JC. (2018). Ex vivo and in vitro assessment of anti-inflammatory activity of seed β-conglutin proteins from Lupinus angustifolius. Journal of Functional Foods. 40:510-519. doi: 10.1016/j.jff.2017.11.040. Impact Factor (JCR/WOS) 3.197, 33/135 (Q1) Food Science & Technology, 36/86 (Q2) Nutrition & Dietetics
2018 Jimenez-Lopez JC., Foley RC, Brear E, Clarcke VC, Lima-Cabello E, Florido JF, Singh KB, Alché JD, Smith PMC. (2018). Characterization of narrow-leaf lupin (Lupinus angustifolius L.) recombinant major allergen IgE-binding proteins and the natural ß-conglutin counterparts in sweet lupin seed species. Food Chemistry. 244:60-70. Doi: 10.1016/j.foodchem.2017.10.015. Impact Factor (JCR/WOS) 5.399, 7/135 (Q1-D1) Food Science & Technology; 5/71 (Q1-D1) Chemistry, Applied; 10/86 (Q1) Nutrition & Dietetics.
2017 Jimenez-Lopez J.C., Foley RC, Brear E, Clarke VC, Lima-Cabello E, Florido JF, Singh KB, Smith PMC, Alche JD. (2017). Characterization of the differential IgE-binding fea-tures of lupinus angustifolius recombinant major allergen (Lup An 1) for potential uses in "sweet lupin" allergy diagnosis and therapy. Allergy 72(103); doi: 10.1111/all.13247. Impact Factor (JCR/WOS) 6.048, 4/27 (Q1) Allergy; 25/155 (Q1) Immunology.
2017. Rosario Carmona, María José Jimenez-Quesada, Elena Lima-Cabello, José Ángel Traverso, Antonio Jesús Castro, M. Gonzalo Claros, Juan de Dios Alché. S-nitroso- and nitro- proteomes in the olive (Olea europaea L.) pollen. Predictive versus experimental data by nano-LC-MS. Data in Brief. (2017) Dec; 15: 474–477. doi: 10.1016/j.dib.2017.09.058. Impact factor (CiteScore) 0.70 (Q2) Multidisciplinary
2017 Estefanía García-Quirós, Rosario Carmona, Adoración Zafra, M. Gonzalo Claros and Juan de Dios Alché. Identification and in silico analysis of glutathione reductase transcripts expressed in olive (Olea europaea L.) pollen and pistil. Lecture Notes in Computer Science Volume 2017, 10209, 185-195. DOI: 10.1007/978-3-319-56154-7_18. Impact factor (SJR/Scimago) 0.295 (Q2) Computer Science (miscellaneous).
2017. Zafra A, Carmona R, Traverso JA, Hancock JT, Goldman MHS, Claros MG, Hiscock SJ and Alche JD (2017) Identification and Functional Annotation of Genes Differentially Expressed in the Reproductive Tissues of the Olive Tree (Olea europaea L.) through the Generation of Subtractive Libraries. Front. Plant Sci. 8:1576. doi: 10.3389/fpls.2017.01576. Impact Factor (JCR/WOS) 3.678, 24/222 (Q1) Plant Sciences.
2017 María José Jimenez-Quesada, Rosario Carmona, Elena Lima-Cabello, José Ángel Traverso, Antonio Jesús Castro, M. Gonzalo Claros, Juan de Dios Alché. Generation of nitric oxide by olive (Olea europaea L.) pollen during in vitro germination and assessment of the S-nitroso- and nitro-proteomes by computational predictive methods. Nitric Oxide Biology and Chemistry 2017. 68:23-37 doi: 10.1016/j.niox.2017.06.005 Impact Factor (JCR/WOS) 4.367, 65/292 (Q1) Biochemistry & Molecular Biology.
2017 Rosario Carmona, Macarena Arroyo, María José Jiménez-Quesada, Pedro Seoane, Adoración Zafra, Rafael Larrosa, Juan De Dios Alché and Manuel Gonzalo Claros. Automated identification of reference genes based on RNA-seq data. BioMed Eng OnLine 2017, 16(Suppl 1):65 DOI 10.1186/s12938‐017‐0356‐5. Impact Factor (JCR/WOS) 1.676, 51/78 (Q3) Engineering, Biomedical.
2017. Elena Lima-Cabello, Victor Alché, Rhonda C. Foley, Sofianos Andrikopoulos, Grant Morahan, Karam B. Singh, Juan D. Alché, Jose C. Jimenez-Lopez. Narrow-Leafed Lupin (Lupinus Angustifolius L.) β-Conglutin Proteins Modulate the Insulin Signaling Pathway as Potential Type 2 Diabetes Treatment and Inflammatory-Related Disease Amelioration. Molecular Nutrition and Food Research 61(5). 2017 Feb 13. doi: 10.1002/mnfr.201600819. Impact Factor (JCR/WOS) 5.151, 5/133 (Q1-D1) Food Science & TEchnology.
2016. Rosario Carmona, Pedro Seoane, Adoración Zafra, Juan de Dios Alché and Gonzalo Claros. Automatic workflow for the identification of constitutively-expressed genes based on NGS reads mapping. Lecture Notes in Computer Science 2016, 9656, 403-414. DOI: 10.1007/978-3-319-31744-1_36. Impact factor (SJR/Scimago) 0.339 (Q2) Computer Science (miscellaneous).
2016 Adoración Zafra, José Carlos Jimenez-Lopez, Rosario Carmona, Gonzalo Claros, Juan de Dios Alché. Transcriptome-based identification of a seed olive legumin (11S globulin). Characterization of subunits, 3D modelling and molecular assessment of allergenicity. Lecture Notes in Computer Science 2016. 9656, 59-70. DOI: 10.1007/978-3-319-31744-1_6. Impact factor (SJR/Scimago) 0.339 (Q2) Computer Science (miscellaneous).
2016 Maria Isabel Covas; Sara Biel; Maria Dolores Mesa; Rafael de la Torre; Juan Antonio Espejo; Juan Ramón Fernández-Navarro; Montserrat Fitó; Estefania Sánchez-Rodríguez; Carmen Rosa; Rosa Marchal; Juan de Dios Alche; Manuela Exposito; Manuel Brenes; Beatriz Gandul; Miguel Angel Calleja. The NUTRAOLEOUM Study, a randomized controlled trial, for achieving nutritional added value for olive oils. BMC Complementary and Alternative Medicine. 2016; 16: 404. Published online 2016 Oct 22. doi: 10.1186/s12906-016-1376-6. Impact Factor (JCR/WOS) 2.288, 6/25 (Q1) Integrative & Complementary Medicine.
2016 Emilia Wilmowicz, Kamil Frankowski, Agata Kucko, Michał Świdzińskic, Juan de Dios Alché, Anna Nowakowska, Jan Kopcewicz. The influence of abscisic acid on ethylene biosynthesis pathway in the functioning of flower abscission zone in Lupinus luteus. Journal of Plant Physiology. 2016. 206:49-58. DOI: http://dx.doi.org/10.1016/j.jplph.2016.08.018 Impact Factor (JCR/WOS) 3.121, 37/211 (Q1) Plant Sciences.
2016. Adoración Zafra, Juan David Rejón, Simon J. Hiscock, Juan de Dios Alché. Patterns of ROS accumulation in the stigmas of Angiosperms and visions into their multi-functionality in plant reproduction. Frontiers in Plant Science. Special Topic: Recent insights into the double role of hydrogen peroxide in plants. Front Plant Sci. 2016; 7: 1112. Published online 2016 Aug 5. doi: 10.3389/fpls.2016.01112 Impact Factor (JCR/WOS) 4.298, 20/211 (Q1-D1) Plant Sciences.
2016. Jose Carlos Jimenez, Elena Lima, Juan de Dios Alche. Ole e 13 is the unique food allergen in olive: structure-functional, substrates docking, and molecular allergenicity comparative analysis. Journal of Molecular Graphics and Modelling (2016) 66:26-40. http://dx.doi.org/10.1016/j.jmgm.2016.03.003. Impact Factor (JCR/WOS) 1.932, 45/104 (Q2) Computer Science, 20/56 (Q2) Mathematical & Computational Biology; 58/79 (Q3), Biochemical Research Methods; 227/289 (Q4) Biochemistyry & Molecular Biology; (datos de 2014).
2016 Jimenez-Quesada MJ, Traverso JA, Alché JD. NADPH oxidase dependent superoxide production in plant reproductive tissues. Research Topic: “Interplay between NO signalling, ROS and the antioxidant system in plants”. Frontiers in Plant Science, vol. 7 number 00359. 2016. DOI=10.3389/fpls.2016.00359. Impact Factor (JCR/WOS) 4.298, 20/211 (Q1-D1) Plant Sciences.
2016 Jose Carlos JIMENEZ-LOPEZ, Adoración ZAFRA, Lucía PALANCO, José Fernando FLORIDO, Juan de Dios ALCHÉ. Identification and assessment of the potential allergenicity of 7S-like SSPs in the olive (Olea europaea L.) seeds. BioMed Research International. Volume 2016 (2016), Article ID 4946872, 11 pages. http://dx.doi.org/10.1155/2016/4946872 Impact factor (JCR/WOS) 2.476, 65/158 (Q2), Biotechnology & Applied Microbiology; 65/128 (Q3) Medicine, Research & Experimental.
2015. Rosario M. Carmona, Adoración Zafra, Pedro Seoane, Antonio J. Castro, Darío Guerrero-Fernández, Trinidad Castillo-Castillo, Ana Medina-García, Francisco M Cánovas, José Aldana-Montes, Ismael Navas-Delgado, Juan De Dios Alché and M. Gonzalo Claros. ReprOlive: a Database with Linked Data for the Olive Tree (Olea europaea L.) Reproductive Transcriptome. Front Plant Sci. 2015 Aug11;6:625. DOI: 10.3389/fpls.2015.00625. Impact Factor (JCR/WOS) 4.495, 15/209 (Q1-D1) Plant Sciences.
2015 Jose C. Jimenez-Lopez, PhD; Agnieszka Zienkiewicz; Krzysztof Zienkiewicz; Juan D. Alche; Maria I. Rodriguez-Garcia. Biogenesis of protein bodies during legumin accumulation in developing olive (Olea europaea L.) seed. Protoplasma (2016) 253(2):517-530. doi: 10.1007/s00709-015-0830-5. Impact factor (JCR/WOS) 2.870, 44/211 (Q1) Plant Sciences, 109/189 (Q3) Cell Biology.
2015 María José Jiménez-Quesada, Jose Ángel Traverso, Adoración Zafra, Jose C. Jimenez-Lopez, Rosario Carmona, M. Gonzalo Claros and Juan de Dios Alché (2015). Identification and in silico Analysis of NADPH Oxidase Homologues Involved in Allergy from an Olive Pollen Transcriptome. Lecture Notes in Computer Science 2015, 9043, 450-459. DOI:10.1007/978-3-319-16483-0_44. Impact factor (SJR/Scimago) 0.369 (Q2) Computer Science (miscellaneous).
2015. Jose C. Jimenez-Lopez, Elena Lima-Cabello, Su Melser, Rhonda C. Foley, Karam B. Singh and Alché JD. (2015). Lupin Allergy: Uncovering Structural Features and Epitopes of ß-conglutin Proteins in Lupinus Angustifolius L. with a Focus on Cross-allergenic Reactivity to Peanut and other Legumes. Lecture Notes in Computer Science 2015, 9043, 96-107. DOI: 10.1007/978-3-319-16483-0_10. Impact factor (SJR/Scimago) 0.369 (Q2) Computer Science (miscellaneous).
2015. Adoración Zafra, Rosario Carmona, José C. Jimenez-Lopez, Amada Pulido, M. Gonzalo Claros, and Juan de Dios Alché. Identification of Distinctive Variants of the Olive Pollen Allergen Ole e 5 (Cu,Zn Superoxide Dismutase) throughout the Analysis of the Olive Pollen Transcriptome. Lecture Notes in Computer Science Volume 2015, 9043, 460-470. DOI:10.1007/978-3-319-16483-0_45. Impact factor (SJR/Scimago) 0.369 (Q2) Computer Science (miscellaneous).
2015 Zienkiewicz K, Alché JD, Zienkiewicz A, Tormo A, Castro AJ, Identification of olive pollen allergens using a fluorescence-based 2D multiplex method. Electrophoresis. 36(7-8):1043-1050. doi: 10.1002/elps.201400508. (2015) Impact Factor (JCR/WOS) 2,482, 26/75 (Q2) Chemistry Analytical; 38/77 (Q2) Biochemical Research Methods.
2014 Noela Prado, Juan de Dios Alché, Juan Casado-Vela, Salvador Mas, Mayte Villalba, Rosalía Rodríguez, and Eva Batanero. Nanovesicles are secreted during pollen germination and pollen tube growth: a possible role in fertilization. Molecular Plant 7(3):573-577. doi: 10.1093/mp/sst153.
2014 Agnieszka Zienkiewicz, Krzysztof Zienkiewicz, Fernando Florido, Francisco Manuel Marco, Pedro José Romero, Antonio Jesus Castro, Juan de Dios Alché. Chip-based CE standardization of olive pollen extracts for allergy diagnosis and therapy. Electrophoresis 35:2681–2685.
2014 Zienkiewicz A, Zienkiewicz K, Rejón JD, Alché Jde D, Castro AJ, Rodríguez-García MI. Olive seed protein bodies store degrading enzymes involved in mobilization of oil bodies. J Exp Bot. Jan;65(1):103-15. doi: 10.1093/jxb/ert355.
2013 Soleimani, A., Morales, S., Jiménez-López, J.C., Castro A.J., Rodríguez-García, M.I. and Alché, J.D. Differential expression and sequence polymorphism of the olive pollen allergen Ole e 1 in two Iranian cultivars. Iranian J of Allergy, Asthma and Immunology 12(1):18-28
2013 Raúl Huertas, Lourdes Rubio, Olivier Cagnac, María Jesús García-Sánchez, Juan De Dios Alché, Kees Venema, José Antonio Fernández & María Pilar Rodríguez-Rosales. The K+/H+ Antiporter LeNHX2 Increases Salt Tolerance by Improving K+ Homeostasis in Transgenic Tomato. Plant Cell and Environment. 36(12):2135-2149 doi: 10.1111/pce.12109.
2013 H. Ribeiro, S. Morales, C. Salmerón, A. Cruz, L. Calado, M. I. Rodríguez-García, J.D. Alché, I. Abreu. Analysis of the pollen allergen content of twelve olive cultivars grown in Portugal. Aerobiologia 29:513-521. May 3. DOI 10.1007/s10453-013-9300-8
2013 Castro A.J., Suárez C., Zienkiewicz Z., Alché J.D., Zienkiewicz A., Rodríguez-García M.I.. Electrophoretic profiling and immunocytochemical detection of pectins and arabinogalactan proteins in the olive pollen during germination and pollen tube growth. Annals of Botany 112(3):503-513. doi:10.1093/aob/mct118
2013 Jimenez-Lopez J.C., Rodriguez-Garcia M.I., Alche J.D. Analysis of the effects of polymorphism on pollen profilin structural functionality and the generation of conformational, T- and B-cell epitopes. PLOS ONE Oct 17;8(10):e76066. doi: 10.1371/journal.pone.0076066.
2013 Rejón J.D., Delalande F., Schaeffer-Reiss C., Carapito C., Zienkiewicz K., Alché J.D., Rodríguez-García M.I., Van Dorsselaer A., Castro A.J.). Proteomics profiling reveals novel proteins and functions of the plant stigma exudate. Journal of Experimental Botany 64(18):5695-5705 doi: 10.1093/jxb/ert345
2013 Jose C. Jimenez-Lopez, Simeon O. Kotchoni, Maria C. Hernandez-Soriano, Emma W. Gachomo, Juan D. Alche. Structural functionality, catalytic mechanism modeling and molecular allergenicity of phenylcoumaran benzylic ether reductase, an olive pollen (Ole e 12) allergen. Journal of Computer-Aided Molecular Design 27(10):873-895. DOI 10.1007/s10822-013-9686-y
2013 Corpas FJ, Alché JD, Barroso JB. Current overview of S-nitrosoglutathione (GSNO) in higher plants. Front Plant Sci. May 8;4:126. doi: 10.3389/fpls.2013.00126.
2013 Jose Angel Traverso, Amada Pulido, María Isabel Rodríguez-García, Juan de Dios Alché. 2013. Thiol-based redox regulation in sexual plant reproduction: new insights and perspectives. Front Plant Sci. Nov 14;4:465. doi: 10.3389/fpls.2013.00465.
2012 Sonia Morales, Antonio J Castro, José Carlos Jiménez-López, Fernando Florido, María Isabel Rodríguez-García, Juan de Dios Alché. A novel multiplex method for the simultaneous detection and relative quantification of pollen allergens. Electrophoresis 33:1367-1374. DOI: 10.1002/elps.201100667.
2012 Jose C. Jimenez-Lopez, Sonia Morales, Antonio J. Castro, Dieter Volkmann, María I. Rodríguez-García & Juan de D. Alché. Characterization of profilin polymorphism in pollen with a focus on multifunctionality. PLoS ONE 7(2): e30878. doi:10.1371/journal.pone.0030878.
2012 Martin Potocký, Přemysl Pejchar, Małgorzata Gutkowska, María José Jiménez Quesada, Andrea Potocká, Juan de Dios Alché Ramírez, Benedikt Kost and Viktor Zárský. NADPH oxidase activity in pollen tubes is affected by calcium ions, signaling phospholipids and Rac/Rop GTPases. J. Plant Physiology 169:1654-1663
2012 Jose C. Jimenez-Lopez; Simeon O. Kotchoni; María I. Rodrigez-Garcia; Juan D. Alche. Structure and functional features of olive pollen pectin methylesterase using homology modeling and molecular docking methods. Journal of Molecular Modeling Dec;18(12):4965-4984. DOI: 10.1007/s00894-012-1492-2.
2012 Chouhra Talbi, Montserrat Argandoña, Manuel Salvador, Juan D. Alché, Carmen Vargas, Eulogio J. Bedmar and María J. Delgado. Burkholderia phymatum improves salt tolerance of symbiotic nitrogen fixation in Phaseolus vulgaris. Plant and Soil. 367(1-2):673-685
2012 A. Zafra, M.J. Jiménez-Quesada, J.A. Traverso, F.J. Corpas, M.I. Rodríguez-García, and J.D. Alché. Peroxisomal localization of CuZn superoxide dismutase in the male reproductive tissues of the olive tree. Microscopy and Microanalysis. 18(S5):33-34. DOI: http://dx.doi.org/10.1017/S1431927612012822
2011 Zienkiewicz, A., Jiménez-López, J.C.; Zienkiewicz, K; Alché, J.D. Rodríguez-García, M.I. Development of the cotyledon cells during olive (Olea euroapea L.) in vitro seed germination and seedling growth. Protoplasma 248(4):751-765.
2011 Zienkiewicz, K., Rejón, J.D., Suárez, C., Castro, A.J., Alché, J.D. and Rodríguez-García. M.I. Whole-organ analysis of calcium behavior in the developing pistil of olive (Olea europaea L.) as a Tool for the Determination of Key Events in Sexual Plant Reproduction. BMC Plant Biology 2011, 11:150.
2010 Castro AJ, Bednarczyk A, Schaeffer-Reiss C, Rodríguez-García MI, Van Dorsselaer A, Alché JD. Screening of Ole e 1 polymorphism among olive cultivars by peptide mapping and N-glycopeptide analysis. Proteomics 10(5):953-62
2010 Krzysztof Zienkiewicz, Antonio J. Castro, Juan de Dios Alché, Agnieszka Zienkiewicz, Cynthia Suárez and María Isabel Rodríguez-García. Identification and localization of a caleosin in the olive (Olea europaea L.) pollen during in vitro germination. J. Exper. Bot. 61(5):1537-1546.
2010 Zafra A, Rodríguez-García M.I., Alché, J.D. Cellular localization of ROS and NO in olive reproductive tissues during flower development. BMC Plant Biology 2010, 10:36 (24 February 2010).
2010 Fendri M., Trujillo M., Trigui, A., Rodríguez-García M.I and Alché J.D. Simple Sequence Repeat identification and endocarp characterization of olive tree accessions in a Tunisian germplasm collection. HortScience 45(10):1429-1436.
2009 Teruel M, Cabrero J, Perfectti F, Alché JD, Camacho JPM. Abnormal spermatid formation in presence of the parasitic B24 chromosome in the grasshopper Eyprepocnemis plorans. Sexual Development 3:284–289 (2009).
2008 AbdelMounim Hamman-Khalifa, Antonio J. Castro-López, José C. Jiménez-López, María I. Rodríguez-García and Juan de D. Alché. Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen. BMC Plant Biology 2008, 8:10.
2008 S. Morales, J.C. Jiménez-López, A.J. Castro, M.I. Rodríguez-García & J.D. Alché. Olive pollen profilin (Ole e 2 allergen) co-localizes with highly active areas of the actin cytoskeleton and is released to the culture medium during in vitro pollen germination. J. Microscopy-Oxford. 231(2) 332-342.
2007 Miguel A. Matilla, Juan L. Ramos, Estrella Duque, Juan de Dios Alché, Manuel Espinosa-Urgel and María Isabel Ramos-González. Temperature and pyoverdine-mediated iron acquisition control surface motility of Pseudomonas putida. Environmental Microbiology 9(7), 1842-1850
2007 Wei Wang , Juan de Dios Alché and Marìa Isabel Rodríguez-García. Characterization of olive seed storage proteins. Acta Physiologiae Plantarum. 29(5): 439-444
2007 JD Alché, AJ Castro, JC Jiménez-López, S Morales, A Zafra, AM Hamman-Khalifa* and MI Rodríguez-García. Differential characteristics of the olive pollen from different cultivars and its biological and clinical implications. J Invest Allerg Clin Immunol 17(Suppl. 1 «Trends in olive pollen allergy»):69-75
2007 Castro AJ, Alché, JD, Calabozo B, Hamman-Khalifa AM, Rodríguez-García MI and Polo F: The Pla l 1 allergen from English plantain (P. lanceolata) pollen shares common epitopes and similar ultrastructural localization with the olive (O. europaea L.) pollen major allergen Ole e 1. J Invest Allerg Clin Immunol 17 (Suppl 1«Trends in olive pollen allergy»):93-99
2006 Alché, J.D., Jiménez-López, J.C., Wang, W., Castro, A.J. and Rodríguez-García, M.I.: “Biochemical characterization and cellular localization of 11S-type storage proteins in olive (Olea europaea L.)” J. Agric. Food Chemistry 54, 5562-5570
2005 P. Barral, C. Suárez, E. Batanero, C. Alfonso, J.D. Alché, M.I. Rodríguez, M. Villalba, G. Rivas and R. Rodríguez: An olive pollen protein with allergenic activity, Ole e 10, defines a novel family of carbohydrate-binding modules and is potentially implicated in pollen germination. Biochemical J. 390, 77-84
2004 Mesa, S., Alché, J.D., Bedmar, E. and Delgado, M.J.: “Expression of nir, nor and nos denitrification genes from Bradyrhizobium japonicum in soybean root nodules”. Physiologia Plantarum 120, 205-211. Impact factor 2.017, 31/138 (Q1) Plant Sciences
2004 Alché, J.D., M’rani-Alaoui M., Castro, A.J. and Rodríguez-García, M.I.: "Ole e 1, the major allergen from olive (Olea europaea L.) pollen, is newly synthesized and released to the culture medium during in vitro germination” Plant Cell Physiol 45(8), 1149-1157.
2003 Alché, J.D., Cismondi, I.A., Castro, A.J., Hamman-Khalifa, A.M. and Rodríguez-García, M.I.: “Temporal and spatial gene expression of Ole e 3 allergen in olive (Olea europaea L.) pollen.”. Acta Biol. Cracov. Bot. 45, 89-96
2003 Castro, A. J., Alché, J.D. Cuevas, J., Romero, P.J., Alché, V. and Rodríguez-García, M.: “Pollen from different olive tree cultivars contains varying amounts of the major allergen Ole e 1”. Int. Arch. Allergy Clin Immunol 131, 164-173.
2002 Alché, J.D., Castro, A.J. and Rodríguez-García, M.I.: “Localization of transcripts corresponding to the major allergen from olive pollen (Ole e I) by electron microscopic non-radioactive in situ RT-PCR”. Micron. 33, 33-37
2001 Wei, W., Alché, J.D., Castro, A.J. and Rodríguez-García, M.I.: "Characterization of seed storage proteins and their synthesis during seed development in Olea euroapea". Int. J. Dev. Biol. 45: 63-64.
2000 Alché, J.D., Castro, A.J., Solymoss, M., Barnabas, B and Rodríguez-García, M.I.: “Cellular approach to the study of androgenesis in maize anthers: immunocytochemical evidence of the involvement of the ubiquitin degradative pathway in androgenesis induction”. J. Plant Physiol. 156(2), 146-155.
2000 Alché, J.D., Castro, A.J., Butowt, R and Rodríguez-García, M.I.: “Ubiquitin and ubiquitin-conjugated proteins in the olive (Olea europaea) pollen”. Sex. Plant Reprod. 12, 285-291.
1999 Alché, J.D. Paul, E. And Dickinson, H.G.: “Heterogously expressed polypeptide from the yeast meiotic gene HOP1 binds preferentially to yeast DNA". Prot. Express. Purif. 16, 251-260.
1999 Alché, J.D., Castro, A.J., Olmedilla, A., Fernández, M.C., Rodríguez, R., Villalba, M. and Rodríguez-García, M.I.: “The major olive pollen allergen (Ole e I) shows both gametophytic and sporophytic expression during anther development, and its synthesis and storage takes place in the RER. J. Cell Sci. 112(15), 2501-2509. 1999.
1998 Alché, J.D., and Dickinson, H.G.: "Affinity chromatographic purification of antibodies to a biotinylated fusion protein expressed in E. coli". Prot. Express. Purif. 12:138-143.
1998 Alché, J.D., Corpas, F.J., Rodríguez-García, M.I. and del Río, L.A.: "Identification and immunolocalization of superoxide dismutase isoenzymes of olive pollen". Physiol. Plantarum 104, 772-776.
1998 Münster, A., Majewska-Sawka, A. and Alché, J.D.: "Identification of arabinogalactan-proteins (AGPs) in sugar beet suspension cultures". Folia Morphol. (Warsz.) 57(1):43.
1997 Butowt, R.; Rodríguez-García, M.I.; Alché, J.D. and Gorska-Brylass, A.: “Calcium in electron-dense globoids during pollen grain maturation in Chlorophytum elatum R.Rb.”. Planta 203: 413-421.
1997 Olmedilla, A.; Alché, J.D. and Rodríguez-García, M.I.: “Nucleolar evolution and coiled bodies during meiotic prophase in Olea europaea: differential localization of nucleic acids.” Eur. J Cell Biol. 74:181-189.
1997 Alché, J.D. and Rodríguez-García, M.I.: "Fluorochromes for detection of callose in meiocytes of olive (Olea europaea L.)". Biotechnics & Histochemistry 72(6):285-290.
1996 Fernández, M.C., Olmedilla, A., Alché, J.D., Palomino, P., Lahoz, C. and Rodríguez-García, M.I.: “Immunogold probes for light and electron microscopic localization of Ole e I in several Oleaceae pollens”: J. Histochem. Cytochem. 44(2):151-158. 1996.
1995 Rodriguez-Garcia, M.I., Fernandez, M.C. and Alché, J.D.: "Immunocytochemical localization of allergenic protein (Ole e I) in the Endoplasmic Reticulum of the developing pollen grain of olive (Olea europaea L.)". Planta 196:558-563.
1995 Rodriguez-Garcia, M.I., Fernandez, M.C., Alché, J.D. and Olmedilla, A.: "Endoplasmic reticulum as a storage site for allergenic proteins in pollen grains of several Oleaceae". Protoplasma 197:111-116.
1994 Alché, J.D., Fernández, M.C. and Rodríguez‑García, M.I.: "Cytochemical features common to nucleoli and cytoplasmic nucleoloids of Olea europaea meiocytes: detection of rRNA by in situ hybridization". J. Cell Sci. 107:621-629.
1994 Martin-Orozco, E., Cardaba, B., del Pozo, V., de Andres, B., Villalba, M., Gallardo, S., Rodriguez-Garcia, M.I., Fernandez, M.C. Alché, J.D., Rodriguez, R., Palomino, P. and Lahoz, C.: "Ole e I: Epitope mapping, cross-reactivity with other Oleaceae pollens and ultrastructural localization". Int. Arch. Allergy Immunol. 104:160-170.
1991 Camacho, J.P.M., Cabrero, J., Viseras, E., López‑León, M.D., Navas‑Castillo, J. and Alché, J.D.: "G banding in two species of grasshopper and its relationship to C, N, and fluorescence banding techniques. Genome 34:638‑643.
1991 Sato, S., Jones, K., Alché, J.D. and Dickinson, H.G.: "Cytoplasmic nucleoloids of Lilium male reproductive cells contain rDNA transcripts and share features of development with nucleoli". J. Cell Sci.100:109‑118.
1989 Alché, J.D. and Rodríguez‑García, M.I.: "Application of X‑Ray microanalysis, diffraction and cytochemical techniques in the study of the structure and chemical composition of inclusions in Olea europaea leaves". Inst. Phys. Conf. Ser. 98: 759‑762.
1988 Alché, J.D. and Rodríguez‑García, M.I.: "Ultrastructural and cytochemical observations on intranuclear inclusions in Olea europaea". Inst. Phys. Conf. Ser. 93 (3): 65‑66.
1987 Cabrero, J.; Alché, J.D. and Camacho, J.P.M.: "A test for equilocality of supernumerary heterochromatin distribution in grassopphers". Genome 29:110‑115.
1987 Cabrero, J.; Alché, J.D. and Camacho, J.P.M.: "Effects of B chromosomes on the activity of nuclear organizer regions in the grassoppher Eyprepocnemis plorans: Activation of a latent nuclear organizer region on a B chromosome fused to an autosome. Genome 29:116‑121.
